Stimulation of yeast mating hormone activity by synthetic oligopeptides
نویسندگان
چکیده
منابع مشابه
Stimulation of yeast mating hormone activity by synthetic oligopeptides.
The biological activities of two synthetic oligopeptides (His-Trp-Leu-Gln-Leu and Trp-Leu-Gln-Leu), which represent part of the primary structure of the mating hormone alpha factor from Saccharomyces cerevisiae, were studied. The peptides did not exhibit hormonal activity by themselves. However, both intensified the mating-type-specific inhibitory effect of native alpha factor on the division o...
متن کاملInhibition of cathepsin D by synthetic oligopeptides.
A number of synthetic oligopeptides with the COOH terminus D-amino acid situated third from the potentially cleavable phenylalanyl-phenylalanyl bond, typified by < Glu-D-Phe-Pro-Phe-Phe-Val-D-Trp (Peptide VI) were shown to be potent competitive inhibitors of cathepsin D and pepsin. Peptide VI, which forms an equimolar nonproductive enzyme l inhibitor complex, inhibited the hydrolysis of methyl[...
متن کاملStimulation of Enzyme Activity by Parathyroid Hormone
Received for publication 26 October 1976 and in revised form 24 January 1977. INTRODUCTION The fat cell adenylate cyclase is a plasma membrane-bound enzyme which is supposed to play a central role in the stimulation of lipolysis by hormones (1). The rat fat cell enzyme reacts to a variety of hormones including catecholamines and peptide hormones such as ACTH, glucagon, and secretin (2-4). By co...
متن کاملSynchronous mating in yeast.
Homogeneous a and alpha unbudded yeast cells in logarithmic phase, grown in supplemented minimal medium and isolated by zonal gradient centrifugation, are used for mating. When these cells are resuspended in aerated defined medium, highly synchronous mating rapidly occurs. Within 20 min of incubation at 30 degrees early sexual pairing is evident; extensive agglutination is observed by 60 min, a...
متن کاملYeast Mating Type
Saccharomyces cerevisiae, baker’s yeast, can exist in either a haploid (1n) or a diploid (2n) state. Haploids express one of two mating types, a or a (alpha). Haploids of either mating type are capable of mating with haploids of the opposite mating type in response to the action of mating pheromones. Mating results in fusion of haploid cells, forming an a/a diploid, which is no longer capable o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1977
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.132.1.28-35.1977